SEQUENCING AND HOMOLOGOUS ANALYSIS OF THE SEXUAL STAGE PFS25 GENE FROM PLASMODIUM FAL CIPARUM PFD-3/YN ISOLATE

VernacularTitle:恶性疟原虫云南株PFD-3／YN Pfs25基因的测序及同源性分析
Author: Youhong WANG ; Xinbing YU ; Haifeng CHEN ; Xuerong LI ; Shuhong LUO
Publication Type:Journal Article
Keywords: Plasmodum falciparum; pfs25; Nucleotide homology
From: Chinese Journal of Zoonoses 2000;(6):19-20
CountryChina
Language:Chinese
Abstract: Malaria vaccines are being developed against different stages in the parasite's life cycle. Although not directly protective ,the sexual stage vaccines would induce antibodies that would prevent infection of mosquitoes when ingestedin a bloodmeal containing sexual stage parasites. pfs25 has been tested to be an important candidate antigen for malarial transmission blocking vaccine . In this report we analyzed the complete code of pfs25 gene of Plasmodium falciparum PFD-3/YN isolate. The result shows that the gene encoding pfs25 of PED-3/YN isolate has a mutant which generates a PstI endonuclease restriction site and shares 99.2％ nucleotide homology with that of NF4 isolate.