CONSTRUCTION OF THE TARGETING VECTORS OF HEAT SHOCK PROTEIN 86 REVF PLASMODIUM FALCIPARUM
10.3969/j.issn.1002-2694.2000.06.007
- VernacularTitle:恶性疟原虫热休克蛋白86(HSP86)基因置换型和插入型打靶载体的构建
- Author:
Lin YE
;
Xinbin YU
;
Jin XU
- Publication Type:Journal Article
- Keywords:
Gene knockout Heat shock protein 86;
Plasmodium falciparum
- From:
Chinese Journal of Zoonoses
2000;(6):21-23
- CountryChina
- Language:Chinese
-
Abstract:
AimIn order to investigate the function of hsp86 gene in Plasmodium falciparum,two fragments of heat shock protein 86 gene of Plasmodium falciparum were amplified and the targeting vectors were constructed. MethodsCulturing the Plasmodium falciparum in vitro,extracting the genome DNA, amplifing the targeted gene with PCR ,contructing the target vector and identifing with restricted enzymes and sequencing. ResultsThe relative fragments were amplified successfully. The insertion and replcement vector with hsp86 gene were constructed. ConclusionConstructing successfully the replacement and insertion vector which is used to the gene knockout in Plasmodium falciparum.
