In vitro culture of human embryonic striatum-derived neural stem cells**★
10.3969/j.issn.2095-4344.2013.27.016
- VernacularTitle:人胚胎纹状体来源神经干细胞的体外培养**★
- Author:
Mingchao FAN
;
Qiaoling WANG
;
Ke LIU
;
Xin ZHANG
;
Yunqian GUAN
;
Peng SUN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2013;(27):5048-5056
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Neural stem cells are always derived from animals, and unsuitable for human transplantation treatment. OBJECTIVE: To explore the in vitro culture methods of human embryonic striatum-derived neural stem cells, and to observe the biological characteristics. METHODS: The human embryonic striatum were separated from the embryo at a gestational age of 8-16 weeks that received induction of labor with water bag, and then the embryonic striatum was in vitro cultured in the serum-free Dulbecco’s modified Eagle’s medium. The cells were passaged after neurospheres formation, and then the cells were induced to differentiation with the Dulbecco’s modified Eagle’s medium/F12 containing 10% fetal bovine serum. RESULTS AND CONCLUSION: The in vitro cultured human embryonic striatum-derived neural stem cells grew rapidly and could express nestin. Colony formation assay showed the cel clone formation rate was 6.0%-7.0%. 5-Bromodeoxyuridine incorporation assay showed the cel proliferation rate was 37.9%. Immunofluorescence staining showed that the cells after induction and differentiation could express Tuj-1, glial fibril ary acidic protein and nestin, but not express myelin basic protein. The results indicate that human embryonic striatum-derived neural stem cells cultured in the serum-free medium can maintain their biological characteristics and have self-renewal capacity, and the cells can differentiate into the neurons and astrocytes induced by the fetal bovine serum.
