Preparation of lentivirus silencing SND1 and its influence on breast cancer cell proliferation and invasion
10.3969/j.issn.1000-8179.2013.13.001
- VernacularTitle:沉默SND1重组慢病毒制备及其对乳腺癌细胞增殖和侵袭的影响*
- Author:
Lin YU
;
Yanbo DI
;
Jie YANG
- Publication Type:Journal Article
- Keywords:
breast cancer;
lentivirus;
staphylococcal nuclease domain containing 1;
proliferation;
invasion
- From:
Chinese Journal of Clinical Oncology
2013;(13):749-753
- CountryChina
- Language:Chinese
-
Abstract:
Objective: This work aimed to construct stable MCF-7 cell sublines from which staphylococcal nuclease domain con-taining 1 (SND1) expression was interfered to analyze the effect of SND1 silencing on the proliferation and metastasis of MCF-7 cells. Methods: The lentivirus that could mediate SND1 silencing was prepared. MCF-7 cells were infected with the lentiviruses to construct stable sub-cell lines. Quantitative real-time polymerase chain reaction and Western blot analysis were employed to determine SND1 ex-pression level. MTS, wound healing, and transwell assays were applied to analyze the effect of SND1 silencing on the proliferation, mi-gration, and invasion of MCF-7 cells. Results: A lentivirus expression vector that contains sequences encoding shRNAs targeting SND1 and an shRNA negative control were successfully established. The lentiviruses (LV-SH1, LV-SH2, LV-SH3, and 和 LV-NC) were then collected and packaged. Stabilized MCF-7 sublines were prepared through infection with lentiviruses. The most efficient MCF-7 stable cell subline, MCF-SH3, was selected for SND1 silencing. Compared with the control cell, the proliferation, migration, and inva-sion potential of MCF-SH3 were significantly decreased. Conclusion: SND1 could promote the proliferation, migration, and invasion of breast cancer cells. Thus, silencing SND1 expression will inhibit such proliferation, migration, and invasion. These results indicated that the unusual expression of SND1 is associated with breast cancer and may participate in cancer progression by affecting prolifera-tion, migration, and invasion.
