MiR-150 downregulation and its significance in mantle cell lymphoma
10.3969/j.issn.1000-8179.2013.13.004
- VernacularTitle:miR-150在套细胞淋巴瘤中的表达及意义
- Author:
Xinwei ZHANG
;
Fang WANG
;
Xiubao REN
;
Jianguo TAO
- Publication Type:Journal Article
- Keywords:
mantle cell lymphoma;
miR-150;
c-Myc
- From:
Chinese Journal of Clinical Oncology
2013;(13):763-766
- CountryChina
- Language:Chinese
-
Abstract:
Objective: This study explores the miR-150 expression and its clinical significance in mantle cell lymphoma (MCL). Methods: The miR-150 and c-Myc expression was measured in 29 primary MCL tissue samples and 7 normal donors through quantita-tive real-time polymerase chain reaction. MiR-150 expression was detected in Mino and HBL-2 cells after c-Myc was knocked down by small interfering RNAs. MiR-150 was analyzed in tet-treated P493-6 cells with Myc turned off. The number of tumor colonies in the BL-2 cells transfected with pre-miR-150 was determined through colony formation assay, and c-Myb was detected through Western blot. Results: Compared with the normal donors, miR-150 expression was significantly downregulated and c-Myc was considerably overexpressed in MCL. Moreover, MCLs with high Myc expression had a significantly low miR-29 expression. MiR-150 was upregu-lated in the Mino and HBL-2 cells with c-Myc knockdown. MiR-150 was evidently upregulated in P493-6 cells after c-Myc was turned off. MiR-150 overexpression suppressed colony formation and c-Myb expression of HBL-2. Conclusion: MiR-150 is downregulated in MCL, which may be related to c-Myc overexpression. The recovery of miR-150 suppresses MCL cell survival. These results indicate that miR-150 may be a potential therapeutic target of MCL.
