Antimicrobial resistance and BRO genotyping of Moraxella catarrhalis isolates from respiratory tract in children
10.3969/j.issn.1000-3606.2013.08.005
- VernacularTitle:儿童呼吸道卡他莫拉菌分离株耐药性与BRO基因分型研究
- Author:
Pinjia WANG
;
Chengbin XIE
;
Yulu WU
- Publication Type:Journal Article
- Keywords:
Moraxella catarrhalis;
antimicrobial resistance;
genotyping
- From:
Journal of Clinical Pediatrics
2013;(8):719-722
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate antimicrobial resistance and beta-lactamase production of Moraxella catarrhalis isolates from respiratory tract in children and to understand the characteristics of BRO beta-lactamase gene. Methods From June 2011 to Sep-tember 2012, 401 Moraxella catarrhalis isolates were obtained from respiratory tract in children. Minimum inhibitory concentrations (MIC) of commonly-used antibiotics were determined by microbroth dilution assay, and beta-lactamase production was detected by Nitroceifn disk test. PCR combining restriction endonuclease analysis was employed to do the BRO genotyping. Results 96.5%iso-lates were beta-lactamase positive (387/401), MIC (MIC50/MIC90) values and resistant rates of beta-lactamase producing isolates were higher than those of non beta-lactamase producing isolates for ampicillin, cefaclor and cefuroxime (P<0.05). The positive rate of BRO gene was 99.2%in beta-lactamase producing isolates (384/387), consisting of 93.0%BRO-1 isolates and 7.0%BRO-2 isolates. MIC50 and MIC90 values of BRO-1+isolates were higher than those of BRO-2+isolates for ampicillin, cefaclor, cefuroxime and azithromycin. Conclusions The beta-lactamase production rate is high in Moraxella catarrhalis isolates from respiratory tract in children. BRO-1 type was the dominant genotype of beta-lactamase producing isolates, having more inlfuence than BRO-2 type in the inlfuence on some beta-lactams and macrolides.
