Sequence analysis of envelope genes in dengue viruses from Fujian Province, 2004-2010
10.3969/cjz.j.issn.1002-2694.2012.10.002
- VernacularTitle:福建省2004-2010年登革病毒E蛋白基因序列分析
- Author:
Meng HUANG
;
Yongjun ZHANG
;
Meiqing LIN
;
Jinzhang WANG
;
Yansheng YAN
;
Yuwei WENG
- Publication Type:Journal Article
- Keywords:
dengue fever;
envelope protein;
phylogenetic tree;
Bayesian;
MrBayes
- From:
Chinese Journal of Zoonoses
2012;(10):973-977
- CountryChina
- Language:Chinese
-
Abstract:
Since several dengue viruses (DENV) have been isolated in Fujian Province during the past decade, sequencing and evolution analyses of viral envelope genes are helpful in determining their possible transmission origins. In this study, viral RNA was extracted from 12 DENV strains from Fujian between 20042010. Viral envelope genes were amplified, cloned into TA vectors and sequenced, and the sequence data were subsequently analyzed by bioinformatics software. Full-length E genes of DENV-1 or DENV-2 of 1 485 bp, and DENV- 3 of 1 479 bp were obtained. It was indicated, from BLAST analysis and phylogenetic trees, that DENV strains in Fujian Province during 20042010 shared the highest similarity with Southeast Asian strains, suggesting that DENV circulating in Fujian Province between 20042010 were probably imported from Southeast Asia. Hence, extensive monitoring on passengers from this region at the entry-ports should be strengthened.
