Characteristic of glycoprotein gene sequences of rabies virus in Guizhou Province
10.3969/cjz.j.issn.1002-2694.2012.07.009
- VernacularTitle:贵州省狂犬病病毒糖蛋白基因序列特征分析
- Author:
Shijun LI
;
Dingming WANG
;
Qing TANG
;
Xiaoyan TAO
;
Hao LI
;
Yan ZHUANG
;
Jingzhu ZHOU
;
Yue WANG
;
Kecheng TIAN
;
Guangpeng TANG
- Publication Type:Journal Article
- Keywords:
rabies virus;
glycoprotein gene;
homogeny;
phylogenetic tree;
Guizhou Province
- From:
Chinese Journal of Zoonoses
2012;(7):689-694
- CountryChina
- Language:Chinese
-
Abstract:
To study the characteristic of glycoprotein gene sequence of rabies virus in Guizhou Province in recent years and provide scientific basis for effective control and prevention of rabies,RT-PCR assay were used to detect human and dog brain tissues derived from different prefectures of Guizhou.The amplification products were sequenced and analyzed with bioinformatics software.The results showed that the full-length G gene sequences of 8 positive samples were obtained by RT-PCR amplification,sequencing and splicing.The homology of eight G gene sequences from Guizhou Province were between 87.4％ -99.9％ and 83.3.％- 100％ on nucleotide and deduced amino acid level,respectively,and the highest homology were found with the genotype 1 strains ( 86.5 ％- 87.0％ for nucleotide and 83.3 ％- 100 ％ for amino acid) among genotype 1- 7 representative strains.Besides,phylogenetic analysis based on the G gene indicated that the relationship of 8 strains derived from Guizhou were closest to genotype 1 Lyssavirus,and the strains of Guizhou were very close to the strains come from Hubei,Hunan,Anhui,Guangxi,Jiangsu provinces and Shanghai,except for GZ01 and GZ09.Moreover,GZ09 were evolutionarily closed to the strains come from Malaysia and Thailand,while the remaining sequences were closed to the strain of Indonesia.This study confirmed on the G gene level that rabies virus epidemic strains circulated in Guizhou Province in recent years belonged to rabies virus genotype 1 and the evolutionary relationship with reported strains come from different provinces of China and different countries were revealed in this study.It will provide scientific basis for effective control and prevention of human and animal rabies in Guizhou Province.
