Establishment of LC-MS/MS method for the determination of forsklin in rat plasma and its pharmacokinetics
10.3969/j.issn.1674-0440.2012.02.010
- VernacularTitle:液相色谱-串联质谱法测定大鼠血浆中forsklin的浓度及其药代动力学
- Author:
Dianwei SONG
;
Decai WANG
;
Zhiyun MENG
;
Ruolan GU
;
Meihui SHI
;
Zhuona WU
;
Jingze WANG
;
Guifang DOU
- Publication Type:Journal Article
- Keywords:
forsklin;
LC-MS/MS;
pharmacokinetics
- From:
Journal of International Pharmaceutical Research
2012;(2):149-153
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the determination of forsklin in rat plasma.Methods After extraction with methyl tert-butyl ether,chromatographic separation was performed on a C18 column with the mobile phase consisting of water ( 0.1％ formic acid)-acetonitrile in a gradient elution mode.A tandem mass spectrometer equipped with electrospray ionization (ESI) source was used as detector in the positive ion mode.Quantification was performed using multiple reaction monitoring (MRM) with the precursor product combination ions of m/z 411→375.3 and 285→193 for forsklin and diazepam.Results Good linearity was obtained in the 0.5-1000 ng/ml range for the analyte and the analytical method was validated in terms of specificity,precision,accuracy,recovery,stability and matrix effect.These assays gave RSD values always lower than 14.4％ and RE values between -3.5 ％ and 3.8％.In addition,the specificity,extraction recovery,stability and matrix effect were satisfactory.Conclusion Due to its high sensitivity,specificity and simplicity,the method could be used for pharmacokinetic studies of forsklin.
