Establishment of an in vitro system evaluating neurotoxicity using neural differentiation of human ES cells and amniotic fluid stem cells
10.3969/j.issn.1000-3606.2011.02.001
- VernacularTitle:利用人胚胎干细胞和人羊水干细胞的神经分化模型建立体外神经毒性测试的实验研究
- Author:
Junmei ZHOU
;
Shengli ZHANG
;
Fang CHEN
- Publication Type:Journal Article
- Keywords:
human embryonic stem cells;
human amniotic fluid stem cells;
neural differentiation;
neurotoxicity
- From:
Journal of Clinical Pediatrics
2011;(2):101-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective A lot of drugs have side effects on the central nerves system. Especially in children. In vivo neurotoxicity tests are time-consuming and expensive. The neural differentiation of human embryonic stem cells and amniotic fluid stem cells provides all ideal in vitro system that Can be applied to evaluate neurotoxicity of drugs. This study was to try to establish such a system. The kainie acid was selected to test the neurotoxicity. Methods The human embryonic stem cells and amniotic fluid stem cells were indueed to differentiate into neural cells by a chemically defined neural induction medium. The induced neural cells were propagated in the presence of basic fibroblast growth factor. Immunocytochemical staining Was applied to confirm these cells' neural identity. The induced cells were propagated under different concentration of kainic acid, then the gosh curve were made based on the cell numbers. Results Both of the human embryonic stem cells and amniotic fluid stem cells could be efficiently induced to be differentiated into neural cells. The neural differentiation efficiency of human embryonic stem cells is higher than that of human amniotic fluid stem cells. The kainic acid has neurotoxieity to the indueed neural cells. Conclusions The neural differentiation of human embryonic stem cells and amniotic fluid stem cells were proved to provide a rapid and convenient approach for estimating the neurotoxlcity of drugs.
