Human Immunodeficiency Virus-Infected T Cells Are Selectively Killed by Monoclonal Anti-gp120 Antibody Coupled to Pokeweed Antiviral Protein.
- Author:
Mi Ran KANG
;
Sun young KIM
;
Yoon Kyu KIM
;
Hyo Jeong HONG
;
Myung Hwan CHO
;
Hyung Sik SHIN
- Publication Type:In Vitro ; Original Article
- MeSH:
Antigens, Viral;
Cell Line;
Glycoproteins;
HIV;
HIV-1;
HIV-2;
Homicide;
Humans*;
Immunotoxins;
Phytolacca americana*;
T-Lymphocytes*
- From:Journal of the Korean Society of Virology
1998;28(4):383-391
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
A murime monoclonal antibody (mAb) specific for the envelope glycoprotein gp120 of human immunodeficiency virus type-I (HIV-1) was chemically coupled to pokeweed antiviral protein (PAP) from Phytolacca americana. The immunotoxin was purified by FPLC using 5200 colum. The purified immunotoxin efficiently bound to HIV-infected T cells as evidenced by fluorescence-activated cell sorter analysis. The immunotoxin selectively killed human T lymphoid lines infected with HIV-lIIIB at less than 250 pM of the immunotoxin cells, while PAP or mAb alone did not have any significant effect on infected cells. The uninfected control T cell lines were not affected. Human cells infected with HIV-2 or other HIV-1 strains were not killed, suggesting that the killing depends completely on the antibody used for coupling. These in vitro results suggest that the PAP-mAb conjugate may be used to selectively remove cells expressing viral antigens from individuals infected with HIV.
