Construction of cRGD-iron oxide nanoparticles and its application in the tumor diagnosis by magnetic resonance imaging
10.3781/j.issn.1000-7431.2010.04.003
- VernacularTitle:cRGD-氧化铁纳米粒的构建及应用于核磁共振成像诊断中的动物研究
- Author:
Yongmei DING
;
Caicun ZHOU
;
Yinmin ZHAO
;
Wei LI
;
Shuyan MENG
- Publication Type:Journal Article
- Keywords:
Carcinoma;
Nanotechnology;
Magnetic resonance imaging;
Early diagnosis
- From:
Tumor
2010;(4):277-282
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct superparamagnetic iron oxide nanoparticles targeting tumor angiogenesis and evaluate their potential value as contrast agent in magnetic resonance imaging (MRI) .Methods:Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles targeting tumor angiogenesis were prepared by using co-precipitation chemical method. Cyclic RGD(cRGD) containing the sequence of Arg-Gly-Asp were conjugated USPIO nanoparticles by using chemical conjugative method to prepare superparamagnetic imaging agent targeting tumor angiogenic vessles. The physical and chemical properties of cRGD-USPIO nanoparticles were detected. The specific binding capabilities of cRGD-USPIO and USPIO to human lung adenocarcinoma cells (A549) and human umbilical vein endothelial cells (HUVEC) were tested by Prussian blue staining. A549 xenografts were established in nude mice, then USPIO and cRGD-USPIO were injected though tail vein, and the MRI signal enhancement effect of cRGD-USPIO was evaluated.Results:We successfully prepared the cRGD-USPIO nanoparticles. Its core diameter was 5-10 nm and the average diameter was (43.97±10.10) nm and the quality saturation magnetic intensity was 59.94 A·m~2·kg~(-1). Cell-binding test suggested that cRGD-USPIO group showed strengthened positive staining. In vivo MRI experiments showed that signals of tumor were significantly reduced in cRGD-USPIO group than that in USPIO group (P<0.01). Conclusion:The constructed cRGD-USPIO nanoparticles can be developed as a potential tumor-specific MRI contrast agent for the early diagnosis of cancer.
