Effect of momordin in inhibiting proliferation and inducing apoptosis of multidrug-resistant K562/A02 cells and its molecular mechanism

VernacularTitle:苦瓜蛋白在K562/A02细胞生长抑制和凋亡中的作用及其机制研究
Author: Lihui YIN ; Shudao XIONG ; Aifang YE ; Yixiang HAN ; Shenghui ZHANG ; Jianbo WU
Publication Type:Journal Article
Keywords: Leukemia; Momordin; Mulidrug resistance-associated proteins; Apoptosis; Cell,K562/A02
From: Tumor 2010;(4):288-292
CountryChina
Language:Chinese
Abstract: Objective:To study the molecular mechanism for momordin in inducing apoptosis of multidrug-resistant human chronic leukemia K562/A02 cells. Methods:The growth inhibition value of K562/A02 cells was detected by CCK-8 method. Cell apoptosis was analyzed by Annexin Ⅴ flow cytometry (FCM) and cell morphological examination. FCM was also used in determining expression of P-glycoprotein, p53 protein, bcl-2 protein and caspase activity. Results:Momordin inhibited the proliferation of K562/A02 cells in a dose-dependent manner. It also induced cell apoptosis, reduced the expression of P-glycoprotein, p53 protein and bcl-2 protein, and increased caspase-3 and caspase-8 activity.Conclusion:Momordin reversed the inhibition of apoptosis in multidrug-resistant K562/A02 cells. The molecular mechanism may be related with down-regulation of expression of p53 protein, P-glycoprotein, and bcl-2 protein and up-regulation of caspase-3 and caspase-8 activities.