Antiproliferation of human colon carcinoma cell line(SW480)by Compound Changtai Granule
- VernacularTitle:复方肠泰抑制人结肠癌SW480细胞增殖的实验研究
- Author:
Shu ZHANG
;
Mingqin SHEN
;
Yongfang DING
;
Xiaoning WANG
- Publication Type:Journal Article
- Keywords:
Compound Changtai Granule;
human colon carcinoma cell line SW480;
cell Cycle;
cell proliferation;
apoptosis
- From:
Chinese Traditional Patent Medicine
2010;(3):374-378
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To check the antiproliferative and apoptosis-inducing effect of Compound Changtai Granule(Radix et Rhizoma ginseng,Semen coicis,Rhizoma curcumae,etc)(CCG)on human colon carcinoma cell line(SW480).METHODS:Methy thiazolyl tetrazolium chromatomerry was used to observe the influence of several concentration of CCG on SW480 cell proliferation cultured in vitro at the various times,and flow cytometry was applied to examining the change in SW480 cell proliferation cycle and apoptosis rate.RESULTS:CCG could successfully prevent SW480 cell from entering G_0/G_1 phase and G_2/M phase,decrease cell numbers in S phase at the same time.CONCLUSION:CCG has an obvious dose-response relationship in the range of 3.51 to 7.81 mg/mL.
