HPLC determination of aflatoxin G_2、G_1、B_2、B_1 in 6 traditional Chinese drugs
- VernacularTitle:脑立清丸等6种中成药中黄曲霉毒素G_2、G_1、B_2、B_1的测定
- Author:
Rong ZHENG
;
Dan MAO
;
Shaomin WANG
;
Yong XU
;
Daoguang ZHANG
;
Ke WANG
;
Shen JI
- Publication Type:Journal Article
- Keywords:
traditional Chinese drugs;
HPLC;
aflatoxins;
immunoaffinity column
- From:
Chinese Traditional Patent Medicine
2010;(3):418-422
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To bring forward a method of determining aflatoxin G_2、G_1、B_2、B_1 in six kinds of traditional Chinese drugs by HPLC.METHODS:After being extracted by 70% methanol,purified by immunoaffinity column,aflatoxins were analysed by HPLC with fluorescence detection.RESULTS:Aflatoxin G_2、B_2 showed a good linear relationship at a range of 1.5-60pg,and Aflatoxin G_1、B_1 at a range of 5-200 pg,r＞0.999 9.The recovery was between 60%-120%.CONCLUSION:The method is simple,accurate and can be used to determine aflatoxin G_2、G_1、B_2、B_1 in Naoliqing Pill,Renshen Yangrong Pill,Rensen Jiapi Pill,Sanqi Tablet,Jinshuibao Capsule and Bailine Capsule.
