Determination of four effective components in Chaihu Shugan Powder by HPLC/DAD
- VernacularTitle:HPLC/DAD测定柴胡疏肝散中4种活性成分的含量
- Author:
Chunyan WANG
;
Danshen ZHANG
;
Wanming ZHANG
;
Chunyan GUO
;
Guiping XUE
- Publication Type:Journal Article
- Keywords:
Chaihu Shugan Powder;
saikosaponin A;
paeoniflorin;
hesperidin;
ferulic acid;
HPLC/DAD
- From:
Chinese Traditional Patent Medicine
2010;(3):422-425
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a method for determining saikosaponin A,paeoniflorin,hesperidin and ferulic acid in Chaihu Shugan Powder(Pericarpium Citri Reticulatae,Radix Bupleuri,Rhizoma Chuanxiong,Fructus Aurantii,Radix Paeoniae alba,etc.).METHODS:The chromatographic saparation was performed on a Hypersil C_(18) column(250 mm×4.6 mm,5 μm).The mobile phase was acetonitril-water(43:57)for saikosaponin A,and the mobile phase for rest components was acetonitrile-0.5% acetic acid(40:60).All of flow rates were 0.8 mL/min and column temperature maintained at 30℃.The detection wavelength was set at 200-400 nm.RESULTS:The four constituents were separated within 15 min.The linear ranges of saikosaponin A,paeoniflorin,hesperidin and ferulic acid were 38.5-166.7 μg/mL(r = 0.999 6),15.9～254.5 μg/mL(r = 0.999 9),22.1-353 μg/mL(r =0.999 3),6.30-201.5 μg/mL(r =0.999 9),respectively.The average recoveries were 97.57%,97.40%,98.86%,96.37%,respectively.The RSD were 2.1%,1.1%,0.70%,1.3%,respectively.CONCLUSION:The method is rapid,simple and accurate,and can be used for quality control of Chaihu Shugan Powder.
