Simultaneous determination of curcumol contentin and β-elemene in Zedoary Turmeric Oil by GC
- VernacularTitle:气相色谱法同时检测莪术油中的莪术醇和β-榄香烯
- Author:
Xing LIU
;
Lan CHENG
;
Guojun LU
;
Shan ZHAO
;
Shenghua WANG
;
Xiaojun MA
- Publication Type:Journal Article
- Keywords:
GC;
Zedoary Turmeric Oil;
curcumol contentin;
β-elemene
- From:
Chinese Traditional Patent Medicine
2010;(3):426-429
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To develop a new method of gas chromatography for simultaneously determining curcumol contentin and β-elemene in Zedoary Turmeric Oil.METHODS:Separation and quantification were achieved on a quartz capillary column(30 m×0.32 mm)of PEG with a column temperature gradient from 80℃(holding for 7 min)to 140℃ at a rate of 20℃/min,holding for 15 min,taking N2 as loader.The temperature for gasification was 210℃;the temperature for detection was 200℃;the compressive stress of column was 0.27 MPa.RESULTS:Under the optimal chromatographic conditions,the linear range of curcumol contentin was within 0.230 5-3.23 mg/mL and the linear range of the β-elemene was within 0.237 5-3.33 mg/mL.The average recovery of curcumol contentin was 100.4% with a relative standard deviation(RSD)of 1.1% and β-elemene was 100.2% with a RSD of 2.2%.CONCLUSION:The results show that it is a simple,rapid,accurate and sensitive method which is suitable for analysis of complex component(curcumol contentin and β-elemene)in Zedoary Turmeric Oil at the same time.
