Determination of cholic acid and muscone in Jawei Xihuang Soft Capsule
- VernacularTitle:加味西黄软胶囊中胆酸、麝香酮的含量测定
- Author:
Fen JIN
;
Fuliang XIONG
;
Dengke XIONG
;
Li XIONG
;
Yang XIANG
- Publication Type:Journal Article
- Keywords:
Jawei Xihuang Soft Capsule;
cholic acid;
muscone;
determination
- From:
Chinese Traditional Patent Medicine
2010;(3):429-432
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To develop a method for determining cholic acid by HPLC-ELSD and GC was applied to determing muscone;in Jawei Xihuang Soft Capsule(Calculus Bovis,Moschus,Venenum Bufonis,Olibanum,Myrrha).METHODS:AC_(18) column(Kromasil C_(18),5 μm,4.6 mm×250 mm)was used as stationary phase,the mobile phase was methanol-0.01% glacial acetic acid(73:21)at a flow rate of 1.0 mL/min.The parameters of ELSD were set as follows:evaporation temperature was 40℃,carrier gas(N_2)pressure was 200 kPa.The GC system consisted of DB-1 capillary column(30 m×0.32 mm×0.25 μm)and FID as the detector.The programmed temperature-GC and internal standard method were employed to determine the content of muscone.RESULTS:The linear ranges of cholic acid and muscone were in the range of 45.2 ng-904 ng and 0.05 mg/mL-0.5 mg/mL respectively.The average recoveries were 99.06% and 99.40% with RSD of 1.56% and 0.95% respectively.CONCLUSION:The method is convenient and accurate,and it can be used for the quality evaluation of Jawei Xihuang Soft Capsule.
