Determination of ferulic acid,rutin and salvianolic acid B in Xiaoshuan Tongluo Capsule by HPLC
- VernacularTitle:HPLC同时测定消栓通络胶囊中阿魏酸、芦丁和丹酚酸B的含量
- Author:
Zhimao DU
;
Anjun HUANG
;
Juan BAI
- Publication Type:Journal Article
- Keywords:
Xiaoshuan Tongluo Capsule;
ferulic acid;
rutin;
salvianolic acid B;
HPLC
- From:
Chinese Traditional Patent Medicine
2010;(3):433-435
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To develop a method for determining the contents of ferulic acid,rutin and salvianolic acid B in Xiaoshuan Tongluo Capsule(Rhizoma Chuanxiong,Radix et Rhizoma Salviale miltiorrhizale,Radix Astragali,Radix et Rhizoma notoginseng,Flos Sophorale,etc.)METHODS:The column was Linksil C_(18)(250 mm×4.6 mm,5 μm).The mobil phase was methanol-acetonitrile-0.1% acetic acid(25:5:70).The detection wavelenght was set at 320 nm.The flow rate was 1.0 mL/min.RESULTS:The calibration curves of ferulic acid,rutin and salvianolic acid B were in good linearity over the ranges of 0.004-0.08 μg,0.432-8.64 μg,0.088-1.76 μg,respectively(r=0.9999).And average recoveries(n=5)were 98.9%,100.1 % and 98.2 %,respectively and RSD were 1.1 %,1.5 %,1.4 %.CONCLUSION:The method is simple,rapid,reliable and can be used for the quality control of Xiaoshuan Tongluo Capsule.
