Determination of purgative constituents in Semen Euphorbiae and Semen Euphorbiae Pulveratun by HPLC
- VernacularTitle:HPLC测定千金子和千金子霜中两种泻下成分的含量
- Author:
Yingxia LI
;
Min YUAN
;
Yongyan CHEN
;
Lijing HOU
;
Xin SHAO
;
Qianqian CI
- Publication Type:Journal Article
- Keywords:
Semen Euphorbiae;
Semen Euphorbiae Pulveratun;
euphorbiasteroid;
lathyrol diadetate benzozte;
HPLC;
determination
- From:
Chinese Traditional Patent Medicine
2010;(3):440-443
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine the contents of euphorbiasteroid and lathyrol diadetate benzozte in Semen Euphorbiae and Semen Euphorbiae Pulveratun from different habitats.METHODS:A kromasil C_(18) column(150 mm×4.6 mm,5 μm)was used with methanol-water(68:32)as the mobile phase and UV detection was at 280 nm.RESULTS:The linear ranges of euphorbiasteroid and lathyrol diadetate benzozte were from 0.200 μg to 1.200 μg and 0.206 μg to 1.651 μg(r = 0.999 7,r = 0.999 9).The contents of euphorbiasteroid and lathyrol diadetate benzozte in Semen Euphorbiae from different habitats ranged from 0.450 8% to 0.625 1 % and 0.692 3% to 0.752 9%.The contents of two kinds of compositions in Semen Euphorbiae Pulveratun ranged from 0.156 8% to 0.221 1% and 0.232 2% to 0.283 0%.CONCLUSION:The method for quantitating euphorbiasteroid and lathyrol diadetate benzozte in Semen Euphorbiae is accurate and reliable,and can be used to control the quality of toxicity Semen Euphorbiae and Semen Euphorbiae Pulveratun.
