HPLC for the simultaneous determination of 13 pesticides in traditional Chinese herbal medicines
- VernacularTitle:高效液相色谱-柱后衍生-荧光检测器测定中药材中13种氨基甲酸酯类农药残留
- Author:
Xiuhong MAO
;
Zhengwei JIA
;
Shui MIAO
;
Jiwei LU
;
Ke WANG
;
Shen JI
- Publication Type:Journal Article
- Keywords:
traditional Chinese medicines;
13 N-methylcarbamate peticides;
HPLC;
post-column derivatization
- From:
Chinese Traditional Patent Medicine
2010;(3):454-459
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study a method for the determination of 13 N-methylcarbamate peticides in traditional Chinese herbal medicines(TCHMs).METHODS:Thirteen pesticides were extracted by acetonitrile,and purified through solid phase extraction(SPE)cartridges,then detected by HPLC with post-column derivatization and Fluorescense Detector excited wavelength was 330 nm,emissive wavelength was 465 nm.RESULTS:The analytical performance was demonstrated by the analysis of 6 TCHMs samples' extracts,spiked at three concentration levels for each pesticide.In general,the recoveries ranging from 74.1 to 108.8%,with relative standard deviations(RSDs)better than 15%,were obtained.The limit of quantification(LOD)of 13 pesticides from 0.000 3 to 0.06 mg/kg.CONCLUSION:The method has good extraction efficiency and purification effect,which could be standard of N-methylcarbamate peticides residues in the routine analysis of TCMs.
