Assay of cinnamaldehyde,costunolide and dehydrocostuslactone in Cinnamomum cassia and Aucklandia lapp by HPLC
- VernacularTitle:HPLC测定肉桂、木香中桂皮醛、木香烃内酯和去氢木香内酯
- Author:
Xiaobin JIA
;
Lijing WANG
;
Yan CHEN
;
Shihua SONG
;
Xuying WANG
- Publication Type:Journal Article
- Keywords:
HPLC;
cinnamaldehyde;
costunolide;
dehydrocostuslactone;
content determination
- From:
Chinese Traditional Patent Medicine
2010;(3):459-462
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish an HPLC method for determining cinnamaldehyde,costunolide and dehydrocostuslactone in the supercritical carbon dioxide extraction of Cinnamomum cassia and Aucklandia lapp.METHODS:The assay was performed on an Agilent HC-C_(18)(150 mm×4.6 mm,5 μm)column by UV detector at the wavelength of 210 nm with acetonitrile-water(gradient elutio)as the mobile phase at the flow rate of 1.0 mL/min,and the column temperature was 30℃.RESULTS:There were good relationships between peak area and sample size of cinnamaldehyde in the range of 148.5-1 732.5 ng,between peak area and sample size of costunolide in the range of 69.42-809.9 ng,and between peak area and sample size of dehydrocostuslactone in the range of 70.32 to 820.4 ng.Average recoveries of them were in turn 99.65%(RSD 0.72%)-99.57%(RSD 1.28%),and 98.90%(RSD 0.81%),respectively.CONCLUSION:The present method is convenient,sensitive and accurate with good reproducibility and can be used for the quality control of the supercritical CO_2 extract of Cinnamomum cassia and Aucklandia lapp.
