HPLC fingerprint of Platycodon grandiflorum in different picking times
- VernacularTitle:不同采收期桔梗HPLC指纹图谱比较研究
- Author:
Xifeng LI
;
Zhe HAO
;
Sumei LIU
;
Yunfeng DU
- Publication Type:Journal Article
- Keywords:
Platycodon grandiflorum;
harvest time;
HPLC;
fingerprints
- From:
Chinese Traditional Patent Medicine
2010;(3):353-356
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish HPLC fingerprints of Platycodon grandiflorum in different picking times in Henan province.METHODS:HPLC chromatography condition:Hypersil C_(18) column(250 mm×4.6 mm,5 μm);the mobile phase was acetonotrile with 0.05% phosphoric acid and the gradient elution mode was applied in chromatographic separation;The flow rate was 0.5 mL/min;The temperature of column was 30℃;the detective wavelength was set at 210 nm;RESULTS:The overall similarity in samples collected in autumn was higher than that in spring,and autumn was the appropriate for harvest.CONCLUSION:This method is simple and accurate with a good reproducibility.It provids a reliable scientific basis for the quality control of Platycodon grandiflorum.
