Cluster analysis of herb active ingredients in reversal effect on HL60/HT
- VernacularTitle:基于聚类分析对中药活性成分逆转HL60/HTM耐药性评价研究
- Author:
Yu CAI
;
Tiange CAI
;
Shaolei YU
- Publication Type:Journal Article
- Keywords:
cluster analysis;
multidrug resistance;
HL60/HT cells
- From:
Chinese Traditional Patent Medicine
2010;(3):379-382
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To do cluster analysis on the herb active ingredients with reversal activity of multi-drug resistance in HL60/HT cells.METHODS:HL60/HT cell was used to check the inhibitory effect of Psoralen,psoralen-A,curcumin,quercetin,psoralidin,isopsoralidin,psoralen phenol,Rhein on cell proliferation by MTT assay.Flow cytometry was used to measure HL fluorescence index and the expression of P-glucose protein.On the basis of the experimental data,herbs were classified as different groups by cluster analysis.RESULTS:Reversion rate,HT concentration and P-gp expression of HL60/HT fell within the range of 6.2 to 12.5,9.6 to 25.6,and 29.3 to 50.1,respectively.CONCLUSION:According to cluster similarity,8 herbal ingredients could be divided into three groups,psoralen,curcumin and quercetin were in the first group.Psoralen-A,psoralidin,isopsoralidin and rhein were in the second group,psoralen phenol was in the third group.
