Influence of Mycobacterium tuberculosis 19-kDa lipoprotein on function and phenotype of macrophage
10.3969/j.issn.1002-2694.2010.03.001
- VernacularTitle:结核分枝杆菌P19对巨噬细胞功能及表型影响的研究
- Author:
Li WEI
;
Qili JIN
;
Yong LIU
;
Peiying XIA
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis P19;
THP-1 cell;
cytokines;
HLA-DR;
phagocytosis
- From:
Chinese Journal of Zoonoses
2010;(3):197-200
- CountryChina
- Language:Chinese
-
Abstract:
To observe the influence of Mycobacterium tuberculosis 19-kDa lipoprotein (Mtb P19) on function and phenotype of macrophage,the Mtb P19 was prepared from cultured Mtb H37Ra and the phorbol myristate acetate-differentiated THP-1 cells were incubated with P19 at the concentration of 10 μg/mL with 5% CO_2 at 37℃ for up to 48 hours.Supernatants were collected for TNF-α and IL-6 detection by ELISA,then the phenotype fluorescent antibodies were stained to analyze HLA-DR expression changes between control group and experimental group.Flow cytometry and microscopy was used to assay the phagocytosis in macrophages stimulated by Mtb P19.IL-6 and TNF-α in collected supernatants were detected.Results indicated that both were found significant increases and their phagocytosis were enhanced.Comparing to the control group,the mean fluorescence intensity showed a significant increase 24hs after stimulation.It presents that Mtb P19 could be able to induce macrophages activation,and it would be significantly important for protection during infection period.
