Expression of Rho GTPaes signaling pathway in non-small cell lung carcinoma and its clinical significance
10.3781/j.issn.1000-7431.2010.03.008
- VernacularTitle:Rho GTPases途径在非小细胞肺癌中的表达及其临床意义
- Author:
Jinchen SHAO
;
Weizhong HE
;
Meiping SHI
;
Min YE
;
Lanxiang ZHAO
;
Jie ZHANG
- Publication Type:Journal Article
- Keywords:
Carcinoma,non-small cell lung;
Reverse transcriptase polymerase chain reaction;
Immunohistochemistry;
Rho GTPases
- From:
Tumor
2010;(3):210-214
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of Rho GTPases signaling pathway in non-small cell lung carcinoma (NSCLC)and its clinical significance.Methods:Molecules of Rho GTPases signaling pathway including RhoC, E-cadherin, MMP-2, and MMP-9 were detected by RT-PCR and immunohistochemistry in 36 specimens of non-small cell lung carcinoma (NSCLC). The relationship between RhoC mRNA and prognosis of patients was evaluated by using Kaplan-Meier survival curve analysis.Results:There was a significant difference in the expression of RhoC mRNA between NSCLC tissues and para-cancerous tissues (P<0.01). The expression of RhoC mRNA in NSCLC was not correlated with gender, age, invasion degree, lymph node metastasis, tumor size, histological classification, and differentiation degree but correlated with different TNM stage (P<0.05). There was a significant positive correlation between the expression of RhoC protein and MMP-2 protein (r=0.474, P=0.003). The survival time of patients with weak expression of RhoC mRNA was longer than those with over-expression of RhoC mRNA, but there was no significant difference between the two groups (P>0.05). Conclusion:Over-expression of RhoC mRNA is closely correlated with the tumorigenesis and progression of NSCLC, and may be related with invasion and metastasis of NSCLC early to middle stage.
