Mechanism of macrophage apoptosis induced by Salmonella typhi
10.3969/j.issn.1002-2694.2010.03.012
- VernacularTitle:伤寒沙门菌诱导巨噬细胞凋亡机制的探讨
- Author:
Tingting LIU
;
Lina MA
;
Fengyun LI
;
Yong LIU
;
Rong FAN
- Publication Type:Journal Article
- Keywords:
Salmonella typhi;
macrophages;
apoptosis;
caspase3;
caspase8;
TNF-α;
NO
- From:
Chinese Journal of Zoonoses
2010;(3):239-242
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the mechanism of macrophages apoptosis induced by stressed Salmonella typhi,macrophages were co-cultivate with inhibitors caspase 3,8,9 and anti-TNF-α antibody and then S.typhi was added to construct the infection model..The rate of macrophage apoptosis was assayed by flow cytometry and the contens of caspase 3.8 ,9 and anti-TNF-α antibody as well as NO were then determined respectively.It was found that the apoptotic rates of macrophages were significantly inhibited by caspase 3 and caspase 8 inhibitors and antibody against TNF-α respectively (P<0.01).A significantly enhanced generation of caspase 3 and caspase 8 activities during macrophage apoptosis induced by S.typhi correlated with the increased generation of TNF-αand NO (P<0.01).These results indicate that the inactive NO and TNF-α mediated inhibitors caspase 3 and caspase 8 participate the exogenous apoptotitic pathway of macrophages induced by S.typhi.
