The sonodynamic effect of chlorin-e6 on the proliferation of human breast cancer cells MDA-MB-231
10.3781/j.issn.1000-7431.2010.03.001
- VernacularTitle:二氢卟吩e6声动力对乳腺癌细胞生长的作用
- Author:
Huijie GAO
;
Weimin ZHANG
;
Xiaohuai WANG
;
Ruinian ZHENG
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Ultrasonic therapy;
Monocytes;
Cell surcvival;
Chlorin-e6
- From:
Tumor
2010;(3):175-179
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the sonodynamic effect of chlorin-e6, a sonosensitizing agent, on the proliferation of MDA-MB-231 cells. Methods:MDA-MB-231 and normal peripheral mononuclear cells (PMNCs) were treated with chlorin-e6 alone or combined with ultrasound. Cell proliferation was determined by MTT assay and cell morphology was observed under inversed fluorescence microscope. Results:Treatment with ultrasound (1.0 MHz, 1.0-2.0 W/cm~2,60 s) or chlorin-e6 (0.10-1.60 mg/mL) alone significantly inhibited the cell proliferation of both MDA-MB-231 and PMNC cells in a intensity-dependent and a dose-dependent manner, respectively. The 50% intensity of ultrasound for inhibiting the growth of MDA-MB-231 and PMNC cells was 1.23 W/cm~2 and 1.25 W/cm~2, respectively (P>0.05) and the IC_(50) of chlorin-e6 was 0.38 mg/mL and 0.77 mg/mL, respectively (P<0.05). Compared with single ultrasound (1.0 MHz,0.5 W/cm~2,60 s) or chlorin-e6 (0.05-0.20 mg/mL) treatment, the combination of ultrasound with chlorin-e6 significantly inhibited the proliferation of MDA-MB-231 cells (P<0.05), while had no synergistic inhibitory effects on the growth of PMNC(P>0.05). Compared with single ultrasound treatment (1.0 MHz, 0.5 W/cm~2,60 s) and single chlorin-e6 treatment (0.20 mg/mL), combination of ultrasound and chlorin-e6 significantly increased the death rate of MDA-MB-231 cells (P<0.05). Conclusion:Ultrasound combined with chlorin-e6 exerted specific inhibitory effect on the proliferation of MDA-MB-231 cells. Chlorin-e6 may be a promising sonosensitizing agent for the treatment of breast cancer.
