Influence of mesothelin silence by siRNA on the growth of human ovarian xenografts in nude mice
10.3781/j.issn.1000-7431.2010.03.002
- VernacularTitle:siRNA介导的间皮素基因沉默对卵巢癌裸鼠腹腔移植瘤生长的影响
- Author:
Dongxiu LI
;
Xiaohua WU
;
Lei ZHANG
;
Li WANG
;
Jin CAO
;
Bojun LIU
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Gene silencing;
Xenograft model antitumor assays;
Mesothelin
- From:
Tumor
2010;(3):180-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the influence of silencing mesothelin(MSLN) gene on the proliferation of ovarian cancer cell line SKOV3 in vitro the growth of human ovarian xenograft in nude mice in vivo.Methods:MSLN-silenced cells (SKOV3-MSLN-shRNA ) and control cells (SKOV3-MSLN-neg) were established after being infected with RNA interference lentivirus and empty vector lentivirus, respectively. SKOV3 cells were used as blank control. Cell proliferation was assayed by clone formation test and cell counting assay. The xenografted model of the three cell lines were established in nude mice. After 2 weeks, the nude mice were sacrificed, and the tumor formation rate, tumor weight, tumor number, and tumor position were recorded. Results:The ability of proliferation of SKOV3-MSLN-shRNA cells was obviously decreased compared with SKOV3-MSLN-neg cells and SKOV3 cells in vitro. The difference was significant (P<0.05). In vivo, the tumor formation rate was 60% in xenografts of SKOV3-MSLN-shRNA cells and 100% in other two groups. The difference was not significant (P>0.05). The average tumor weight, tumor number, and number of tumor position were all decreased in SKOV3-MSLN-shRNA group compared with the two control groups (P<0.05). Conclusion:Silencing MSLN gene decreased the proliferation of ovarian cancer SKOV3 cells in vitro and inhibited the growth of xenografts of ovarian cancer cells in nude mice.
