Study on the action mechanism for δ-tocotrienol-induced apoptosis of human hepatoma HepG2 cells
10.3781/j.issn.1000-7431.2010.03.003
- VernacularTitle:δ-生育三烯酚诱导人肝癌HepG2细胞凋亡的机制研究
- Author:
Zhongquan ZHANG
;
Mei XU
;
Guoqiang HU
;
Songqiang XIE
- Publication Type:Journal Article
- Keywords:
Liver neoplasms,experimental;
Tocotrienols;
Apoptosis;
Caspases;
Mitochondrial membrane potential
- From:
Tumor
2010;(3):184-187
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To elucidate action mechanism of δ-tocotrienol in inducing apoptosis of human hepatoma HepG2 cells. Methods:Cell proliferation and viability were assessed by MTT assay; cell cycle distribution, apoptotic rate and mitochondrial membrane potential were measured by using high content screening system; the expression of apoptosis-related protein such as caspase-3, caspase-8, caspase-9, Bcl-2, Bax, tBid and cytochrome C in the HepG2 cells were evaluated by Western blotting. Results:δ-Toco-trienol inhibited HepG2 cell proliferation and induced apoptosis in a dose-dependent manner. This growth-inhibiting effect of δ-toco-trienol correlated with loss of mitochondrial membrane potential and release of cytochrome C from mitochondria to cytoplasm, and regulation of the protein expression of Bcl-2 family members, such as up-regulation of Bax and tBid and down-regulation of Bcl-2. Subsequently tocotrienol induced the activation of caspase-3, caspase-8, and caspase-9 which finally induced apoptosis of hepatoma HepG2 cells. Conclusion:δ-Tocotrienol induced apoptosis of human hepatoma HepG2 cells via mitochondrial pathway and membrane death receptor pathway.
