Hypoglycemic effect of bone marrow mesenchymal stem cells in vitro differentiated into islet-like cells
10.3969/j.issn.2095-4344.2013.31.019
- VernacularTitle:骨髓间充质干细胞体外诱导分化为胰岛样细胞的降血糖作用*★
- Author:
Liming ZHANG
;
Renhao WANG
;
Meirong WAN
;
Quan WEN
;
Xiaoyun LIU
;
Yong SHAO
- Publication Type:Journal Article
- Keywords:
organ transplantation;
organ transplantation academic discussion;
bone marrow mesenchymal stem cells;
induced differentiation;
co-culture;
islet cells;
cell transplantation;
diabetes;
immunocytochemistry;
dithizone staining;
other grants-supported paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(31):5699-5704
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Islet and islet cel transplantation for the treatment of diabetes has achieved effect, but the research is limited dut to the shortage of islet and immune rejection. OBJECTIVE:To observe the effect of transplantation of islet-like cells that in vitro differentiated from bone marrow mesenchymal stem cells on the treatment of diabetes in rats. METHODS:The rat bone marrow mesenchymal stem cells were induced with basic fibroblast growth factors and hepatocyte growth factors, and then received immunocytochemistry staining to detect the induction. The Sprague Dawley rats received intraperitoneal injection of streptozotocin to establish the diabetes models. After modeling, the rats were randomly divided into control group and experimental group (transplanted with induced islet-like cells). The experimental group was transplanted with the induced islet-like cells through renal capsule, and the control group was transplanted with normal saline in the same dose. The blood glucose and body mass of the diabetes rats were observed after transplantation. RESULTS AND CONCLUSION:The bone marrow mesenchymal stem cells could differentiate into islet-like cells after in vitro induced with basic fibroblast growth factors and hepatocyte growth factors. There was no significant change in blood glucose of the control group after transplantation (P>0.05), and the blood glucose of the rats in the experimental group was significantly decreased compared with the control group (P<0.05). The bone marrow mesenchymal stem cells can differentiate into islet-like cells after in vitro induced with the induction system containing basic fibroblast growth factors and hepatocyte growth factors, and the islet-like cells have a certain ability of insulin secretion. The transplantation of induced islet-like cells after transplanted into the diabetes rats through renal capsule can decrease the blood glucose level of the rats.
