Detection of dengue virus RNA in blood clots by multiplex nested reverse transcription-PCR
10.3969/cjz.j.issn.1002-2694.2012.08.016
- VernacularTitle:多重套式RT-PCR检测患者凝血块中登革病毒RNA
- Author:
Yongjun ZHANG
;
Meng HUANG
;
Yuwei WENG
;
Youxian ZHENG
;
Jinzhang WANG
- Publication Type:Journal Article
- Keywords:
dengue virus;
multiplex nested RT-PCR;
nucleic acid amplification test
- From:
Chinese Journal of Zoonoses
2012;(8):832-836
- CountryChina
- Language:Chinese
-
Abstract:
Dengue is the most common vector borne viral disease of humans globally.Detection of viral RNA from suspected patient specimens is rapid,specific and confirmative in laboratory diagnosis of dengue infections during the acute phase.In this study,a multiplex nested reverse transcription PCR (RT-PCR) system was established for clinical specimens.While other nucleic acid amplification tests showed relatively low sensitivity,the multiplex nested RT PCR assay detected 4 cases among blood clots from 8 serologically confirmed dengue patients.These results suggested that blood clots of dengue patients could be used in laboratory diagnosis,and that the multiplex nested RT PCR assay,which simplified the detection procedure,could facilitate viral RNA detection of specimens in clinical laboratories.
