Dynamics of specific antibody and circulating antigen in serum from the mice infected with Armillifer agkistrodontis

VernacularTitle:尖吻蝮蛇舌形虫感染小鼠血清中特异性抗体和循环抗原的动态观察
Author: Hao LI ; Shaohong CHEN ; Yongnian ZHANG ; Jian GUO ; Zhengshan CHANG ; Jiaxu CHEN
Publication Type:Journal Article
Keywords: Armillifer agkistrodontis; circulating antigen; specific antibody; mice
From: Chinese Journal of Zoonoses 2012;(8):807-810
CountryChina
Language:Chinese
Abstract: In this study,adults of Armillifer agkistrodontis (A.agkistrodontis) were collected from Agkistrodon acutus,and then the eggs were separated to feed mice.In the next step,when the infection model was established,blood serum of infected mice were collected after 1,2 and 3 weeks,respectively.Furthermore,ELISA and dot- ELISA were used to detect the dynamic change of specific antibodies and circulating antigens respectively.The specific antibodies increased from 8th week,reached the top at 12th week,decreased from 16th week,and then maintain at the same level constantly.Meanwhile,the specific antibodies were typed.It is evident that IgM antibody appeared first.However,it was substitute by IgG1 after 16 weeks.Moreover,the circulating antigens have been detected in the 1st week by dot-ELISA.Then,the dilution between 1:8 to 1:128were founded in 3rd week.The highest dilution with 1:256 appeared at 8th week,maintained before 11th week and then decreased gradually,which might provide a significant clinical implication for early diagnosis of circulating antigens.