The study on Norovirus infection status among patients with virus diarrhea in Xiamen district
10.3969/j.issn.1002-2694.2010.03.017
- VernacularTitle:厦门地区病毒性腹泻诺如病毒感染状况
- Author:
Huineng ZHENG
;
Li LI
;
Juanjuan CHEN
;
Shijie HUANG
;
Guilin MA
;
Huixin WEN
;
Litong SHEN
;
Changyi ZHOU
;
Jianwei HUANG
- Publication Type:Journal Article
- Keywords:
Norovirus;
virus diarrhea;
RT-PCR;
ELISA
- From:
Chinese Journal of Zoonoses
2010;(3):259-262
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of the present study was to investigate Norovirus infection status among patients with virus diarrhea in Xiamen city and provide evidence for exploring the prevalence characteristics and constituting appropriate control strategy.From April 2007 to July 2008,323 fecal samples of virus diarrhea cases collected from 3 surveillance hospitals in Xiamen were detected for antigen and RNA by ELISA and Real-Time RT-PCR respectively.The RdPd genes from some samples were furtherly amplified and sequenced for genogroup identification when the Real-Time RT-PCR detection results were positive.In the 323 fecal specimens,68 (21.05%) were positive for Norovirus antigen by ELISA and 107 (31.13%) were positive for Norovirus RNA by Real-Time RT-PCR.The overall positive prevalence rate of Norovirus in Xiamen was 38.08%.107 positive specimens were detected by Real-Time RT-PCR and results showed that 80 strains were Norovirus GGⅡ(74.77%),2 strains were GGⅠ(1.87%) and 25 strains (23.36%)were unidentified.It's indicated that Norovirus infection in Xiamen district was mainly caused by Norovirus GGⅡ,and Norovirus was also the main cause for virus diarrhea.
