Prospective fungal antigen testing in high-risk pediatric patients

VernacularTitle:儿童血液真菌抗原检测的应用研究
Author: Li ZHAO ; Ying WANG ; Yunfang ZHOU ; Biru LI ; Jing CHEN ; Huiliang XUE ; Jingyan TANG
Publication Type:Journal Article
Keywords: invasive fungal infection; (1,3)-β-D-glucan; galactomannan; children
From: Journal of Clinical Pediatrics 2010;(1):1-6
CountryChina
Language:Chinese
Abstract: Objective To assess the diagnostic potential of circulating galactomannan (GM),(1,3)-β-D-glucan (BG), and a combination of both biomarkers among high-risk pediatric patients.Methods Circulating GM antigen was detected by ELISA kits (Platelia~(TM) Aspergillus) and BG antigen by a turbidimetric kinetic method (GKT-5M Set Kinetic Fungus Detection Kit).Positive tests were defined by two consecutive values of GM index ≥0.5 or by a single value ≥0.8, and by BG detection ≥ 10 pg/ml.Results A total of 130 patients were enrolled.Two was identified with proven IFI, twenty probable IFI.Sensitivity, specificity were 81.8%, 82.4% for plasma BG detection, respectively; 75.0%, 94.4% for GM detection, respectively; 50.0%, 96.3% for combined GM and BG detection, respectively.Conclusions Both circulating GM and BG detections are available for most of the common pathogens and demonstrated desirable sensitivity and specificity among pediatric high-risk population.Both assays can be used as prospective screening tools.Combination of detections of both biomarkers would improve specificity for IA diagnosis.