Expression of Beelin-1 and LC3 in adriamycin induced cardiomyopathy rats
10.3969/j.issn.1000-3606.2010.01.022
- VernacularTitle:自噬基因Beclin-1和微管相关蛋白LC3在大鼠阿霉素心肌病中的表达及其意义
- Author:
Jie HUANG
;
Wenhua YAN
;
Haitao LU
;
Ling SUN
;
Qiuqin XU
- Publication Type:Journal Article
- Keywords:
adriamycin;
cardiomyopathy;
Beclin-1;
LC3;
3-MA;
autophagy
- From:
Journal of Clinical Pediatrics
2010;(1):76-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expressions of autophagy-related gene Beclin-1 and LC3 in adriamycin induced eardiomyopathy rats,to prnve that autophagy might take part in the development of adriamycin induced eardiomyopathy in rats,so as to provide experimental and theoretical evidence for preventing and treating adriamycin induced cardiomyopathy.Methods Forty-five male SD rats were randomly divided into 3 groups,control groups,ADR group and ADR+3-MA group.The model of adriamycin induced cardiomyopathy rats was established.The tissue sample taken from the left ventricle wall was checked for the morphons of autophagosome by electron microscope. The expressions of Beelin-1 and LC3 of myocardium were detected.Results The morphons of autophagosome in ADR group was significantly increased compared with that in control and ADR +3-MA groups. The expression of Beclin-1 in myocardium of ADR group was significantly inereased compared with that in control and ADR +3-MA groups (P < 0.05).The level of LC3 in myocardium of ADR group was significantly increased compared with that in control and ADR+3-MA groups (P < 0.05).Conclusions Autophagy plays an important role in adriamycin induced cardiomyopathy.
