Determination of ginsenoside Rb_1,Re,Rb_2 and Rd in Shenqi Granules by microbore liquid chromatography
- VernacularTitle:微管液相色谱法测定参芪颗粒中4种人参皂苷含量
- Author:
Shuang SHOU
;
Jianmin ZHANG
;
Zhongmin YU
;
Kun CHEN
- Publication Type:Journal Article
- Keywords:
ginsenoside Rb_1;
ginsenoside Rc;
ginsenoside Rb_2;
ginsenoside Rd;
microbore liquid chromatography( microore LC )
- From:
Chinese Traditional Patent Medicine
2010;(1):68-71
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To develop a method for determining ginsenoside Rb_1,Rc,Rb_2 and Rd in Shenqi Granules (Radix Rhizoma Ginseng,Radix Astragali,Rhizoma Atractylodis macrocephalae,Poria,etc.) by microbore LC.METHODS: Microbore liquid chromatography was applied.Microsil C_(18) column( 150 mm×1.0 mm) was used with the mobile phase containing acetonitrile-water for gradient elution.The flow rate was 50 μL/min,and the detection wavelength was set at 220 nm.RESULTS : The relationships between the concentrations and the peak areas of these for components were all linear.The recoveries were 98.14% for ginsenoside Rb_1,98.04% for ginsenoside Rc,98.79% for ginsenoside Rb_2,and 97.97% for ginsenoside Rd respectively.The relative standard deviations (RSD) were 0.67%,0.99%,0.75%,and 1.46%,respectively.CONCLUSION: This method is simple,convenient and can be used for quality control.
