RP-HPLC fingerprint of Danhong Injection
- VernacularTitle:注射用丹红(粉针)HPLC指纹图谱研究
- Author:
Jing LIU
;
Xiangguo LIU
;
Guoqiang LI
;
Yi ZHENG
;
Yanqing ZHENG
- Publication Type:Journal Article
- Keywords:
Danhong Injection;
Radix et Rhizoma salviae miltorrhizae;
Hos carthami;
fingerprint;
HPLC
- From:
Chinese Traditional Patent Medicine
2010;(1):10-12
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the fingerprint method for quality control of Danhong sterile powder for injection (Radix et Rhizoma salviae mihiorrhizae,Flos carthami).METHODS:The samples were determined by Agilent 1100 DAD-HPLC with SinoChrom ODS-BP column(250 mm×4.6 mm,5 μm),by gradient elution using methanol-0.1% phosphoric acid,as mobile phase 30 ℃ column temperature.flow rate of 1.0 mL/min,detection wavelength was set at 280 nm,and inject volume 20 μL.RESULTS:Its fingerprint exhibited better separation for each peak,revealing amount of finger information,Based on the ten batches of Danhong sterile powder for injection,according to the technical requirements of fingerprint on injection of Chinese traditional medicine.CONCLUSION:Proposed HPLC fingerprint can be used as the important evidences for the quality control of Danhong Injection.
