Simultaneous determination of two compounds in Mosla chinensis by RP-HPLC
- VernacularTitle:RP-HPLC同时测定不同采收期江香薷两种成分的含量
- Author:
Haowu HU
;
Xiaoming XIE
;
Rengeng SHU
;
Yuesheng WANG
- Publication Type:Journal Article
- Keywords:
Mosla chinensis Maxium;
luteolin;
negletein;
RP-HPLC
- From:
Chinese Traditional Patent Medicine
2010;(1):88-91
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times.METHODS: The Angilent Zorbax,Extend C-18 column(4.6 mm×250 mm,5μm) was used with methanol-water (containing 0.4% phosphonic acid)(50: 50) as mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was set at 350 nm.RESULTS : The method had the good linear relationship within the range of 0.04-0.61 μg for luteolin and within the range of 0.20-3.01 μg for negletein,respectively,with the correlation coefficient of 0.999 9.The average recoveries of the two flavones were 97.32%,98.60%,respectively.The contents of luteolin and negletein in Mosla chinensis Maxium.varied in different picking times,which reached the its maximum at florescence (264.8 μg/g,2 746.3 μg/g) in August,respectively.CONCLUSION: The method is fast,rapid,convenient,accurate and can be used for quality control of Mosla chinensis Maxium.
