Bone marrow mesenchymal stem cells from diabetic versus normal rats for treatment of myocardial infarction
10.3969/j.issn.2095-4344.2013.27.013
- VernacularTitle:糖尿病与正常大鼠骨髓间充质干细胞移植治疗心肌梗死的比较★
- Author:
Zhonglu YANG
;
Huishan WANG
- Publication Type:Journal Article
- Keywords:
stem cells;
stem cel transplantation;
bone marrow-derived stem cells;
myocardial infarction;
cel transplantation;
diabetes;
myocardium;
pathological changes;
cel proliferation;
cel morphology;
stem cel photographs-containing paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(27):5026-5032
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Current studies have shown that bone marrow mesenchymal stem cells from normal or young people usual y serve as a source of transplanted cells in stem cel transplantation treatment of myocardial infarction. OBJECTIVE: To compare the therapeutic effects of bone marrow mesenchymal stem cells from diabetic and normal rats on myocardial infarction. METHODS: Under sterile conditions, bone marrow mesenchymal stem cells from normal and diabetic rats were harvested. Then, rat models of myocardial infarction were established and randomly divided into three groups:100 μL cellsuspension containing 105-106 bone marrow mesenchymal stem cells from F2 normal or diabetic rats was injected into myocardial infarction lesions, and 100 μL Dulbecco’s modified Eagle’s medium containing 20%fetal bovine serum was injected serving as blank control. After 1 month, hematoxylin-eosin staining for myocardial infarction lesions was performed for histomorphological observation. Bcl-2 expression was detected by immunohistochemistry method. RESULTS AND CONCLUSION: Based on cel morphology observation and flow cytometry identification, high-purity bone marrow mesenchymal stem cells could be obtained using rat femoral bone marrow adherent culture. Cel growth curve showed that normal rat bone marrow mesenchymal stem cells grew faster than those from diabetic rats. At 1 month after transplantation, histomorphological improvement was seen in the infarcted area after transplantation of normal rat bone marrow mesenchymal stem cells as compared with the other two groups. In addition, the Bcl-2 expression in the infarcted area was higher in the normal rat cel group than the the other two groups. These findings indicate that bone marrow mesenchymal stem cells from normal rats grow faster than those from diabetic rats, and the cells from normal rats have better therapeutic effects on myocardial infarction.
