Study on the gene of antimicrobitic resistance of Moraxella catarrhalis isolated from lower respiratory tract of children with pneumonia in Chongqing area
10.3969/j.issn.1000-3606.2010.02.006
- VernacularTitle:重庆地区肺炎儿童下呼吸道卡他莫拉菌分离株耐药基因研究
- Author:
Rong LUO
;
Ying HUANG
;
Enmei LIU
;
Lan LIU
;
Yuan CHEN
;
Yonghong YANG
- Publication Type:Journal Article
- Keywords:
children;
Moraxella;
antimierobiol resistance gene
- From:
Journal of Clinical Pediatrics
2010;(2):124-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of resistance to beta-lactam antibiotics of Moraxella catarrhalis (Me) in children with lower respiratory tract infection, to guide the rational and objective administration and provide measures of avoiding changes of antimicrobial resistance of Mc. Methods Total 40 strains Mc with positive beta-lactamase were taken from lower respiratory tract of hospitalized children with pneumonia from July to December in 2006. Polymerase chain reaction technology was used to amplify genes. The DNA product of the 40 strains were digested by restriction enzyme (BCgI). The expression and type of the drug resistance gene (BRO) for ampicillin according to different strap in agarose gel electrophoresis were analyzed. Clinical characteristics of different genotype was studied. Results Among 40 strains Me with positive Nitrocefin disk, positive BRO-1 gene was 90.0% while positive BRO-2 gene was 7.5%, and another 2.5% was neither positive BRO-1 gene nor positive BRO-2 gene. BRO-3 or TEM-1 enzyme produced may be the possible cause. The diameter of inhibition zone to beta-lactam antibiotics of Mc was smaller in positive BRO-1 gene than that Mc of positive BRO-2 gene. Conclusions BRO-1 gene was the main genotype of Mc with beta-laetamase positive in our study.
