Screening of the pORF3 binding protein of HEV in human liver cells by using yeast double hybridization system
10.3969/j.issn.1002-2694.2010.02.004
- VernacularTitle:酵母双杂交筛选人肝细胞文库戊肝病毒pORF3结合蛋白
- Author:
Yanjun KANG
;
Quan SHEN
;
Wen ZHANG
;
Xiuguo HUA
;
Xueqin RAN
;
Jiafu WANG
- Publication Type:Journal Article
- Keywords:
hepatitis E virus;
interacting protein;
yeast double hybridization system
- From:
Chinese Journal of Zoonoses
2010;(2):111-114
- CountryChina
- Language:Chinese
-
Abstract:
HEV is a positive strand RNA virus containing 3 open reading frames(ORFs), while the function of its binding protein pORF3 is poorly understood. In our study, the interactive protein of the pORF3 from human liver cDNA library was screened by the SOS recruitment system, and the construction of the pSOS bait-ORF3 plasmid was identified. The expression of the pORF3 was analyzed by Western blotting, and the detection result of the self activation of bait protein was obtained as expected. Totally, 8 putative interacting proteins had been successively screened. Some of these cellular binding proteins of pORF3, especially the important immunological pathway kinase p110δ, may advance our understanding of the role in ORF3 protein of HEV, and the possible implications of these candidate proteins for the further research are discussed.
