Cloning and prokaryotic expression of the ompA gene of Chlamydia psittaci in cows
10.3969/j.issn.1002-2694.2010.02.011
- VernacularTitle:奶牛流产鹦鹉热衣原体ompA基因的克隆与原核表达
- Author:
Zhuqing SONG
;
Changqing QIU
;
Jizhang ZHOU
;
Xiaoan CAO
;
Guozhen LIN
;
Fuying ZHENG
;
Xiaowei GONG
;
Guanghua WANG
;
Yanming WEI
- Publication Type:Journal Article
- Keywords:
Chlamydia psittaci;
ompA gene;
cloning;
prokaryotic expression
- From:
Chinese Journal of Zoonoses
2010;(2):140-143
- CountryChina
- Language:Chinese
-
Abstract:
The ompA gene of Chlamyia psittaci in cows was amplified by PCR with primers designed based on those reported in GenBank.The amplified ompA gene was inserted into the bacterial plasmid vector pGEX-4T-1 and then transformed into E.coli BL21(DE3) with IPTG induction. The gene was derived from plasmid pMD18-T vector and then sequenced.It was demonstrated that this recombinant fusion protein of approximately 68kD in molecular mass was highly expressed in inclusion body and more pure proteins would be produced after purification.The fusion protein specifically reacted with positive sera of bovine Chlamydia as demonstrated by Western blotting. These results indicate that this recombinant fusion protein shows good reactivity and could be used to develop the diagnostic kit for bovine Chlamydia and genetic engineering vaccine.
