The drug sensitivity of human lung adenocarcinoma stem cells to cisplatin and carboplatin

VernacularTitle:人肺腺癌干细胞对顺铂和卡铂的药物敏感度分析
Author: Jinsu HUANG ; Xuelian QI ; Xueyan ZHANG ; Yan LI ; Baohui HAN ; Qin GENG ; Jin ZHOU ; Huili XU ; Qianggang DONG
Publication Type:Journal Article
Keywords: Lung neoplasms; Tumor stem cells; Drug screening assays,antitumor; Cisplatin; Carboplatin
From: Tumor 2010;(2):95-99
CountryChina
Language:Chinese
Abstract: Objective:To analyze the drug sensitivity of human lung adenocarcinoma stem cells (LASC) to cisplatin (DDP) and carboplatin (CBP). Methods:Human lung adenocarcinomaic cells SPC-A1,AG,and CPA-Y2 were treated with DDP and CBP. The cell viability of cells was detected by CCK-8 assay. The phenotypic characteristics of drug surviving cells(DSCs)were determined by immunofluorescence staining. The LASC population was then separated by magnetic-activated cell sorting method. The LASC in DSCs was traced by using green fluorescence protein (GFP). The drug sensitivity of DSCs to DDP and CBP was analyzed.Results:The LASC exhibited the phenotypes of bronchioalveolar stem cells (BASC, OCT4~+CCSP~+SP-C~+). After mixture of CD221~+LASC with CD221~-lung adenocarcinoma differentiated cells, the DSC population showed OCT4~+BASC phenotypes. These DSCs were significantly resistant to DDP and CBP.Conclusion:LASC has a high resistance to DDP and CBP. This may be the reason for tumor recurrence after chemotherapy.