Pathological changes of VX2 implanted breast cancer ablated with high intensity focused ultrasound in rabbits
10.3781/j.issn.1000-7431.2010.02.004
- VernacularTitle:高强度聚焦超声消融兔VX2种植性乳腺肿瘤的病理学变化
- Author:
Lingchuan NIU
;
Wenbing ZOU
;
Lian ZHANG
;
Hui ZHU
;
Like XIANG
;
Zhibiao WANG
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Ultrasound,high-intensity focused;
Neoplasm seeding;
Proliferating cell nuclear antigen;
Succinate dehydrogenase;
VX2 tumor,rabbit
- From:
Tumor
2010;(2):105-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the pathological changes of VX2 implanted breast cancer ablated by high intensity focused ultrasound (HIFU) in rabbits. Methods:Thirty-six rabbits implanted VX2 breast carcinoma were randomly divided into treatment group (n=24) and control group (n=12) two weeks after implantation. HIFU ablation was performed in the treatment group guided by ultrasound B. The pathological changes were observed under light microscope after hematoxylin-eosin staining and then observed under electron microscope. The activity of succinate dehydrogenase (SDH) was measured. Proliferating cell nuclear antigen (PCNA) was determined by immunohistochemistry. Results:Histopathologic observation found that the morphological features and nucleus karyotypes of tumor cells had no significant changes, but vacuole-like structure appeared in cytoplasm. Typical coagulation necrosis of tumor cells were observed under electron microscope. SDH activity detection showed that tumor cells were inactivated. The expressions of PCNA was positive in control group and negative in treatment group. Conclusion:Electron microscopy, enzyme property test, and immunohistochemistry verified that VX2 implanted breast cancer cells were dead after HIFU ablation in rabbits.
