Proteomic screening of the proteins associated with early lung squamous carcinoma
10.3781/j.issn.1000-7431.2010.02.009
- VernacularTitle:蛋白质组学方法筛选早期肺鳞癌相关蛋白
- Author:
Zhen YAN
;
Guizhi LIU
;
Jianshe WANG
;
Fang ZHOU
;
Wu YAO
;
Weidong WU
;
Yiming WU
- Publication Type:Journal Article
- Keywords:
Lung neoplasms;
Carcinoma,squamous cell;
Proteomics;
Annexin A1;
Heat-shock proteins,small
- From:
Tumor
2010;(2):130-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen the early biomarkers associated with lung carcinoma by using proteomic method. Methods:The proteins of early lung squamous cell carcinoma tissues and adjacent normal tissues were separated by two-dimensional gel electrophoresis (2-DE) and differentially expressed proteins were screened by mass spectrometry (MS). The differential expressions of part proteins were verified by immunohistochemical method. Results:The proteomic profiles of lung squamous cell carcinoma and the adjacent normal tissues were established and the average matched protein spots in the two group were 626 and 602, respectively. Ten protein spots with higher expression in lung squamous cell carcinoma tissues were identified as Annexin 1(Anx-A1),heat shock proteins (HSP27), etc. The proteins were mainly related with cell cycle and signal transduction. The results of immunohistochemistry showed that the positive expression rates of Anx-A1 and HSP27 in lung squamous cell carcinoma tissues were significantly increased (P<0.05). Conclusion:Proteomic technology was an effective method for preliminary identification of the proteins associated with early lung carcinoma. The identified proteins laid a solid foundation for further screening the molecular markers for early diagnosis and treatment of lung cancer.
