K-ras mutations in colorectal cancer at different stages
10.3781/j.issn.1000-7431.2010.02.010
- VernacularTitle:结直肠癌不同病变阶段K-ras基因突变的研究
- Author:
Yongqi SHEN
;
Yunbin YE
;
Xiongwei ZHENG
;
Chao LI
;
Qiang CHEN
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Genes,ras;
Mutation;
DNA mutational analysis
- From:
Tumor
2010;(2):134-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the alteration of K-ras mutations in different stages of colorectal cancer(CRC) and its influence on the progression of CRC. Methods:The 20 paraffin-embedded tissues, including primary foci, metastatic lymph nodes, remoter metastatic foci, colorectal adenoma, and normal colorectal tissues, were collected from 20 patients with colorectal cancer. The sequence of PCR-amplified products were analyzed. Results:The wild K-ras gene was expressed in normal colorectal tissues. The mutation frequency of K-ras gene was 20.0% (4/20) in colorectal adenoma, 30.0% (6/20) in primary foci, 25.0% (5/20) in metastatic lymph nodes, and 30% (6/20) in remote metastatic lesions. In the samples with K-ras mutations, the consistency of the types of K-ras mutations between primary foci and colorectal carcinoma, lymph node metastatic lesions, remote metastatic lesions was 0.0%(0/4), 40.0%(2/5), and 50.0%(3/6), respectively.Conclusion:The colorectal adenoma, metastatic lymph nodes and remote metastatic lesions were not suited for K-ras analysis as routine samples in clinical practice. If the samples of primary lesions were not available, the detection results of metastatic lymph nodes and remote metastatic remote lesions will provide some reference values. K-ras gene had several different mutations in the progression of CRC.
