Ethyl acetate extract from Cortex periplocae induced apoptosis of human esophageal carcinoma cell line TE-13
10.3781/j.issn.1000-7431.2010.01.002
- VernacularTitle:香加皮乙酸乙酯提取物诱导人食管癌TE-13细胞凋亡的作用机制
- Author:
Xiaohui SHANG
;
Xiaoli SHANG
;
Baoen SHAN
;
Yumin CHEN
;
Fengzhi REN
;
Xiaoxia LIU
- Publication Type:Journal Article
- Keywords:
Esophageal neoplasms;
Cortex periplocae;
Plant extracts;
Apoptosis;
TE-13 cells
- From:
Tumor
2010;(1):6-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of ethyl acetate extract from Cortex periplocae (CPEAE) on apoptosis of human esophageal carcinoma cell line TE-13 and to elucidate its mechanism. Methods:Inhibitory effect of CPEAE on TE-13 proliferation was tested by MTT assay. The morphological changes of cell apoptosis were observed by Giemsa staining and transmission electron microscopy. Cell cycle and apoptotic ratio were tested by flow cytometry (FCM). The protein expression of CDK4 was observed by Western blotting.Results:CPEAE inhibited proliferation of TE-13 cells in a concentration-dependent and time-dependent manner, and its IC_(50) value was (2.443±0.005) μg/mL at 48 h (P<0.05). The characteristic morphological changes of apoptosis were observed in TE-13 cells after treatment with CPEAE under transmission microscope. A typical subdiploid peak was detected by flow cytometry. CPEAE decreased the expression of gene CDK4 in TE-13 cells. Conclusion:CPEAE can induce apoptosis of TE-13 cells. The effect is related with down-regulation of CDK4 expression.
