Effects of exogenous NO on cell proliferation and cell cycle of gastric cancer cell line SGC-7901

VernacularTitle:外源一氧化氮对胃癌SGC-7901细胞增殖及细胞周期的影响
Author: Jianrong SANG ; Yongchang CHEN ; Genbao SHAO ; Xiaojia HUANG
Publication Type:Journal Article
Keywords: Stomach neoplasms; Nitric oxide donors; Nitric oxide synthase; Proliferating cell nuclear antigen; Cell cycle
From: Tumor 2010;(1):21-25
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effect of exogenous nitric oxide(NO) on the growth and proliferation of gastric cancer cell line SGC-7901. Methods:The inhibitory effects of NO donor sodium nitroprusside (SNP) and nitric oxide synthase (NOS) inhibitor N-nitro-L-arginine methylester(L-NAME) on the proliferation of SGC-7901 cells were analyzed by MTT assay. The changes of mRNA and protein expression of proliferating cell nuclear antigen(PCNA) and caspase-3 were examined by RT-PCR and Western blotting. The cell cycle was measured using flow cytometry. Results:Compared with control group, more cells in the SNP group were arrested at G_1 and G_0 phases (P<0.05) and fewer cells were at S phases (P<0.05). SNP decreased the speed of cell-cycle progression from G_0/G_1 phase into S phase. SNP inhibited the proliferation of SGC-7901 cells and reduced the mRNA and protein expressions of PCNA and caspase-3. NOS inhibitor L-NAME reversed the effects of SNP. Conclusion:NO inhibited cell growth and proliferation, but accelerated apoptosis of gastric cancer cells.