Relationship between the expressions of ERCC1 and ERCC2 in peripheral venous blood and cancer tissues of esophageal squamous cell carcinoma patients
10.3781/j.issn.1000-7431.2010.01.014
- VernacularTitle:食管鳞状细胞癌患者外周血和肿瘤组织中ERCC1及ERCC2表达的相关性研究
- Author:
Hui ZHANG
;
Zhisong FAN
;
Wei LIU
;
Xianli MENG
;
Baoqing LI
;
Junfeng LIU
;
Guoxiang WU
;
Yong CHEN
;
Jing ZUO
;
Yalei Lü
;
Yudong WANG
- Publication Type:Journal Article
- Keywords:
Esophageal neoplasms;
Reverse transcriptase polymerase chain reaction;
Hematologic test;
Gene,ERCC1,ERCC2
- From:
Tumor
2010;(1):68-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the feasibility of detecting excision repair cross-complementing 1(ERCC1)and ERCC2 in peripheral venous blood instead of cancer tissues from esophageal squamous cell carcinoma patients. Methods:The expressions of ERCC1 and ERCC2 mRNA were detected by using RT-PCR in 39 cases of peripheral venous blood samples, esophageal squamous cell carcinoma tissues, and adjacent normal tissues. ELISA was used to determine the levels of ERCC1 and ERCC2 proteins in serum. The periphe-ral blood from 10 healthy volunteers was used as control. Results:Expression levels of ERCC1 and ERCC2 mRNA and protein were significantly higher in peripheral blood from healthy control than those in esophageal carcinoma patients (P<0.05). There was a positive correlation between the expression of ERCC1 and ERCC2 mRNA in peripheral blood and cancer tissues (P<0.01). Conclusion:The expression levels of ERCC1 and ERCC2 mRNA in peripheral blood can indirectly reflect their expression levels in human esophageal squamous cell carcinoma tissues.
